Fig. 5.
SU5416 and SU5614 inhibition of constitutive STAT5 activation in cells expressing FLT3 ITD.
MV 4-11 (FLT3 ITD) cells were incubated with varying concentrations of inhibitor and stimulated with FL. In this immunoprecipitate using total STAT5 antibody, phosphorylation was ligand-independent but could be inhibited by SU5416 or SU5614 with an IC50 of 100 nM and 10 to 50 nM, respectively. Total STAT5 protein expression was unaffected by treatment.
