Effect of SU5416 and SU5614 on MV 4-11 (FLT3 ITD) and RS 4;11 (FLT3 WT) cell proliferation.

SU5416 and SU5614 inhibit proliferation of MV 4-11 (FLT3 ITD) cells but not RS 4;11 (FLT3 WT) cells. Cells were incubated with inhibitors for 72 hours, and proliferation assessed with an XTT-based assay. Proliferation is expressed as a percentage of vehicle-treated controls (error bars indicate 1 standard deviation). (A) For MV 4-11 (FLT3 ITD), the IC₅₀ was between 100 and 1000 nM for SU5416, while it was approximately 100 nM for SU5614. SU6597 and SU6668 affected proliferation only at the 10-μM dose. (B) RS 4-11 (FLT3 WT) are not dependent on FL for proliferation, and only the highest dose of SU5416 had an effect on proliferation. There was no significant effect of the other compounds on the growth of RS 4;11 (FLT3 WT). These experiments were performed without addition of FL.