Fig. 3.
![Fig. 3. Effects of various Val814 mutants on the factor-independent growth of Ba/F3Val814 cells. / Proliferation of Ba/F3 cells stably expressing KITWT (WT) or various Val814 mutants at various concentrations of rmIL-3 or rmSCF was measured with the [3H]thymidine incorporation assay. Cells transfected with pEF-BOS vector alone (Vector) were used as a negative control. Three independent assays were performed with comparable results, and data from a representative experiment are shown. Each point represents the mean of triplicate samples. Bars represent standard errors. At some data points, the standard error was too small to be shown by bars. Y represents tyrosine (Tyr); F, phenylalanine (Phe).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/3/10.1182_blood-2002-01-0177/4/h80333732003.jpeg?Expires=1735565583&Signature=u4EcaDW~bGiYth4uB16joK2qPjWA1lOmd6jb2hHgiq9R6xLEA-r~9xDYtgUpDff5pYAtLK61QCUdF-sOo-RC4n4nNhwND3xrdezQUV~Zf~Qid3pHfu-BvsSwFdgZLhYuhHSe68L52Jgzq2K8~Ak2illqKjfyAR1SwN0CT18SbZv3O-j3MAB2YiimZtwBQ3fMQuQzUvyICSBQ5-Wlov6aqx-cQsywlFmccSenRUhocN6m9q3I1Wa~3yw3coXzjga1Wk-ID~LRBUxNUAbkbFMT1P~ugpbPYD7WI8-YejkovdgVZ8uBqQDlYK1b2jgMzXRBLqyapCJzYf8iO534ooAqdw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of various Val814 mutants on the factor-independent growth of Ba/F3Val814 cells.
Proliferation of Ba/F3 cells stably expressing KITWT (WT) or various Val814 mutants at various concentrations of rmIL-3 or rmSCF was measured with the [3H]thymidine incorporation assay. Cells transfected with pEF-BOS vector alone (Vector) were used as a negative control. Three independent assays were performed with comparable results, and data from a representative experiment are shown. Each point represents the mean of triplicate samples. Bars represent standard errors. At some data points, the standard error was too small to be shown by bars. Y represents tyrosine (Tyr); F, phenylalanine (Phe).
