Fig. 1.
Fig. 1. Flow cytometric analysis of the expanded peripheral blood B-cell population. / Patient PBMCs were stained for CD19 and CD27 and gated according to light-scatter properties. Propidium iodide and CD19 expression were used to identify viable CD19+ cells. Cells were counterstained for CD20, CD5, CD10, sIgM, sIgκ light chain, and sIgλ light chain, respectively, and were plotted against CD27.

Flow cytometric analysis of the expanded peripheral blood B-cell population.

Patient PBMCs were stained for CD19 and CD27 and gated according to light-scatter properties. Propidium iodide and CD19 expression were used to identify viable CD19+ cells. Cells were counterstained for CD20, CD5, CD10, sIgM, sIgκ light chain, and sIgλ light chain, respectively, and were plotted against CD27.

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