Dose dependence of CDC activity with different anti-CD20 mAbs.

To determine the relative efficacy with which various anti-CD20 mAbs evoked CDC, mAbs were titrated from 10 to 0.08 μg/mL and incubated with EHRB cells for 15 minutes; NHS was added at 20% and assessed for CDC activity as detailed in the legend to Figure 1. As controls, anti-CD37 (WR17) and anti-CD38 (1B4) mAbs were included and assessed in the same manner.