JAQ1, JAQ2, and JAQ3 deplete GPVI in vivo.

(A) Platelet counts from antibody-treated mice were monitored over 10 days after injection of the indicated antibodies (all 100 μg). Results are expressed as mean ± SD of 9 mice/group. (B) Flow cytometric analysis of P-selectin expression and GPIIb/IIIa activation on platelets from JAQ1-, JAQ2-, JAQ3-treated mice or control mice on day 3 after injection. The results shown are representative of 6 mice/group. Similar results were obtained at all other time points tested. (C) Diluted blood collected at the indicated time points after antibody injection was incubated with FITC-labeled JAQ1 (5 μg/mL) for 15 minutes at RT and analyzed directly. Results are presented as mean ± SD of 9 mice/group. (D) Whole platelet proteins were separated by SDS-PAGE under nonreducing conditions and immunoblotted with HRP-labeled JAQ1 (anti-GPVI) or EDL1 (anti-GPIIIa) followed by ECL.