Fig. 4.
In vitro analysis of GPVI-depleted platelets.
(A) Heparinized prp from mice on day 5 after treatment with irrelevant IgG2a or the indicated anti-GPVI mAb was stimulated with collagen, CRP, or ADP and light transmission was recorded. The results shown are representative of 6 mice/group. (B) Whole blood from mice treated with irrelevant IgG2a, JAQ1, JAQ2, or JAQ3 was perfused at wall shear rates of 1000 s−1 (4 minutes) over a collagen-coated surface and representative phase-contrast microscope images were taken (n = 3-6). Original magnification, × 630.
