CD150 and CD28 provide an additive effect in T-cell costimulation of DNA synthesis.
CD150-mediated T-cell costimulation is independent of SAP. (A) Splenic T cells from Balb/c mice were stimulated with plate-bound anti-CD3 (145.2C11, 1 μg/mL) for 72 hours in the presence and absence of anti-mCD150 monoclonal antibodies (10 μg/mL) and anti-CD28 (1 μg/mL, black bars). Proliferation was measured by3H-thymidine uptake during the last 16 hours of culture. Data are representative of at least 3 separate experiments. (B) Splenic T cells from Balb/c (white bars) or SAP−/− mice (black bars) were stimulated with plate-bound anti-CD3 (145.2C11 1 μg/mL) for 72 hours in the presence and absence of 4 different anti-mCD150 monoclonal antibodies (9D1, 4D7, 6C12, 17A4, 10 μg/mL). Proliferation was measured by 3H-thymidine uptake during the last 16 hours of culture. Data are representative of at least 3 separate experiments.
