Fig. 6.
Fig. 6. Anti-CD150 antibodies enhance up-regulation of IL-12Rβ2 mRNA transcripts in CD4 T cells during TH1 polarization. / Real-time RT-PCR for IL-12Rβ2 mRNA transcripts was performed from total RNA from the CD4 T cells in the experiment of Figure 5 as described in “Materials and methods.” CD4 T cells were stimulated under TH1 conditions or TH2 (IL-4 + αIL-12) with or without anti-CD150 (9D1) followed by 48 hours' rest in IL-2 and secondary stimulation with anti-CD3 alone or anti-CD3 plus anti-CD150 as indicated. Relative expression of IL-12Rβ2 was normalized to the level of β2-microglobulin in each sample. 1° and 2° represent primary and secondary stimulation conditions, respectively.

Anti-CD150 antibodies enhance up-regulation of IL-12Rβ2 mRNA transcripts in CD4 T cells during TH1 polarization.

Real-time RT-PCR for IL-12Rβ2 mRNA transcripts was performed from total RNA from the CD4 T cells in the experiment of Figure 5 as described in “Materials and methods.” CD4 T cells were stimulated under TH1 conditions or TH2 (IL-4 + αIL-12) with or without anti-CD150 (9D1) followed by 48 hours' rest in IL-2 and secondary stimulation with anti-CD3 alone or anti-CD3 plus anti-CD150 as indicated. Relative expression of IL-12Rβ2 was normalized to the level of β2-microglobulin in each sample. 1° and 2° represent primary and secondary stimulation conditions, respectively.

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