![Fig. 3. Activity of the transgenes in isolated macrophages from 3 different transgenic mice. / This study generated 3 strains of transgenic mice. They were 3.5fms-EGFP (4 independent lines), 7.2fms-EGFP (6 independent lines), and 7.2fmsΔFIRE-EGFP (6 independent lines). Analyses were done on at least 3 independent lines for each strain. Macrophages isolated from peritoneum (thioglycollate–elicited peritoneal macrophages [TEPM]), derived from bone marrow macrophages (BMMs) and from bronchoalveolar lavage (BAL), were subjected to FACS analysis. Representative FACS profiles (filled histograms) of macrophages isolated from 3.5fms-EGFP TG mice are shown in the top row. Middle row profiles show the representative profiles of EGFP expression in macrophages isolated from 7.2fms-EGFP TG mice; histograms in the bottom row are the representative profiles of EGFP expression in macrophages isolated from 7.2fmsΔFIRE-EGFP TG mice. Dotted lines represent histograms of nontransgenic littermate controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/3/10.1182_blood-2002-02-0569/4/h80333727003.jpeg?Expires=1736045730&Signature=uMsbJE6Uls3gB1gqlV8vvUhMRLD1WBEYiPAo6zfMIA~LKtjPnrqVQp-IQ5QmXKWrzvpeU7IeSeKaxCDopt2KTfG7h0NcdMpZKOs2hvs0D8KoTxz3T0mtExGTArM8XCWHAJ6tgRjYP9vLcbuBdbRpCj~lAbHWe4Hp4mpFHmSesKp13cctlwKY5hXgGysc69k8A2VvyJ66tqDmfDGgKA2eaMGSydPlj---BWbxwo5JHN1u7x0N7DwIL-6FGB7Hhqh-q5zPhi4~Cxm34XhJhTd~e3YNFyv2QR0V2iTKPQNWIDjlLgLky8-vrceCe4pjaty1lhaMNAhuvFClQeRDTRP~rA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activity of the transgenes in isolated macrophages from 3 different transgenic mice.
This study generated 3 strains of transgenic mice. They were 3.5fms-EGFP (4 independent lines), 7.2fms-EGFP (6 independent lines), and 7.2fmsΔFIRE-EGFP (6 independent lines). Analyses were done on at least 3 independent lines for each strain. Macrophages isolated from peritoneum (thioglycollate–elicited peritoneal macrophages [TEPM]), derived from bone marrow macrophages (BMMs) and from bronchoalveolar lavage (BAL), were subjected to FACS analysis. Representative FACS profiles (filled histograms) of macrophages isolated from 3.5fms-EGFP TG mice are shown in the top row. Middle row profiles show the representative profiles of EGFP expression in macrophages isolated from 7.2fms-EGFP TG mice; histograms in the bottom row are the representative profiles of EGFP expression in macrophages isolated from 7.2fmsΔFIRE-EGFP TG mice. Dotted lines represent histograms of nontransgenic littermate controls.
