Expression of EGFP during embryonic development.

(A) In 7.2fms-EGFP TG mice, other than those of macrophage lineage, EGFP is also expressed in trophoblast cells. This panel shows the EGFP expression in cultured trophoblastic giant cells derived from the e6.5 ectoplacental cone. (B) Numerous EGFP⁺ stellate cells were observed in the yolk sac at e9.5. (C) EGFP⁺ cells were observed by whole-mount visualization in the interdigital region of the developing footplate of an e12 to e13 embryo. (D) Sagittal section of an e13.5 embryo showing numerous EGFP⁺ cells in the epidermal layer of the dorsal part of the embryo. (E) Numerous EGFP⁺ cells were observed in the mesenchymal area beneath the chest. (F) The liver and its surrounding area were occupied by a large number of EGFP⁺ cells. EGFP⁺ cells also present in the brain, especially in the pons-midbrain junction (G) and choroid plexus (H). (I) Section trough somites shows the presence of EGFP⁺ cells around the developing vertebrae. (J) Lung also contains EGFP⁺cells. (K) EGFP⁺ cells present in the intrinsic muscle of the tongue. (L) Few EGFP⁺ cells were observed in the developing eye.