Analysis of CD11c⁺ B220⁺ cells in RAG1^−/− mice.

A RAG1^−/− thymic suspension was obtained after collagenase digestion. Four-color staining was performed with anti-F4/80–Cy5, anti-Gr1–PE, anti-CD11c–FITC and anti-B220–CyChrome. Upper plots show that a typical thymic population of CD11c⁺ B220⁺ Gr1⁺F4/80⁻ cells develop in RAG1^−/− mice. Lower contour plots show the characteristic FSC versus SSC profiles of CD11c⁺ B220⁻ and CD11c⁺B220⁻ cells.