Defective DC development from ICSBP^−/− BM cells in vitro.

(A) DCs generated in vitro were stimulated with LPS and analyzed for expression of indicated surface markers. Numbers indicate the percentages of double-positive or single-positive cells. (B) DCs generated in vitro stimulated as described in “Materials and methods” were analyzed for surface marker expression by flow cytometry. Bars in the graphs represent MFI. Results are representative of 3 independent experiments.