Fig. 1.
Fig. 1. Targeting strategy and Southern blot analysis. / (A) Targeting strategy. The genomic organization of the murineEla2 gene is shown in the upper panel. Exons are indicated by solid boxes. The targeting vector is shown in the second panel. The asterisk represents the G→A mutation at nucleotide 298 of the cDNA. The neomycin phosphotransferase gene driven by the phosphoglycerate kinase I (PGK) promoter and flanked by loxP sites is denoted as Neo. Cre-recombinase–mediated excision of theNeo resistance cassette generated the final recombined allele, shown in the bottom panel. The location of the external probe used in the Southern blot analysis and the size of the expectedBamHI fragments are shown. (B) Southern blot analysis ofBamHI-digested genomic tail DNA isolated from the progeny of a heterozygous intercross. The wild-type allele (7.6 kb) and recombined allele (3.6 kb) are shown.

Targeting strategy and Southern blot analysis.

(A) Targeting strategy. The genomic organization of the murineEla2 gene is shown in the upper panel. Exons are indicated by solid boxes. The targeting vector is shown in the second panel. The asterisk represents the G→A mutation at nucleotide 298 of the cDNA. The neomycin phosphotransferase gene driven by the phosphoglycerate kinase I (PGK) promoter and flanked by loxP sites is denoted as Neo. Cre-recombinase–mediated excision of theNeo resistance cassette generated the final recombined allele, shown in the bottom panel. The location of the external probe used in the Southern blot analysis and the size of the expectedBamHI fragments are shown. (B) Southern blot analysis ofBamHI-digested genomic tail DNA isolated from the progeny of a heterozygous intercross. The wild-type allele (7.6 kb) and recombined allele (3.6 kb) are shown.

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