Proliferation responses by OVA-specific CD4+T cells from mice immunized with OVA plus MIP-1α or MIP-1β.
Groups of 5 C57BL/6 mice were nasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (■)or 1.0 μg MIP-1α (▨) or MIP-1β (▪) in PBS. One week after the last immunization, lower respiratory tract (lung and mediastinal lymph nodes)–, Peyer patch–, spleen-, and CLN-derived T cells were purified and cultured at a density of 5 × 106/mL with 500 μg/mL OVA for 3 days with T cell–depleted, irradiated splenic feeder cells (1 × 106/mL) in complete medium. Experimental groups consisted of 5 mice, and studies were repeated 3 times. Proliferation was measured by 3H-thymidine incorporation. The stimulation index corresponds to counts per minute (cpm) of cell cultures containing OVA divided by the cpm of cultures without OVA. The data presented are the mean stimulation indexes ± SEMs of quadruplicate cultures. Asterisks indicate statistically significant differences (P < .05) from the stimulation index of mice immunized with OVA alone.
