UCN-01 and PD184352 interact synergistically to induce apoptosis in MM cells.

Logarithmically growing 8226, H929, and U266 MM cells were exposed to 10 μM PD184352 ± 150 nM UCN-01 for 24 hours, after which cytospin preparations were obtained and apoptosis assessed by TUNEL assay as described in “Materials and methods.” Slides were viewed under fluorescence microscopy under × 60 magnification. An additional experiment yielded equivalent results.