American Society of Hematology

Fig. 7.

$Fig. 7. IL-6 fails to block PD184352/UCN-01–mediated mitochondrial damage despite enhancing Akt phosphorylation. / (A) 8226, H929, and U266 MM cells were exposed to 10 μM PD184352 + 150 nM UCN-01 in the presence or absence of 100 ng/mL IL-6 for 24 hours, after which cells were lysed and Western blot analysis performed to monitor PARP cleavage (whole cell lysates) or cytochrome c and Smac/DIABLO release (S-100 fractions) as described in “Materials and methods.” (B) Alternatively, Western blot analysis was performed to assess the effects of these agents on phosphorylation of Akt and ERK as well as total ERK expression. In each case, lanes were loaded with 30 μg of protein. The results of a representative experiment are shown; a second study yielded equivalent results.$

IL-6 fails to block PD184352/UCN-01–mediated mitochondrial damage despite enhancing Akt phosphorylation.

(A) 8226, H929, and U266 MM cells were exposed to 10 μM PD184352 + 150 nM UCN-01 in the presence or absence of 100 ng/mL IL-6 for 24 hours, after which cells were lysed and Western blot analysis performed to monitor PARP cleavage (whole cell lysates) or cytochrome c and Smac/DIABLO release (S-100 fractions) as described in “Materials and methods.” (B) Alternatively, Western blot analysis was performed to assess the effects of these agents on phosphorylation of Akt and ERK as well as total ERK expression. In each case, lanes were loaded with 30 μg of protein. The results of a representative experiment are shown; a second study yielded equivalent results.

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