Analysis of platelets and PBMCs.

(A) Flow cytometric analysis of platelets. Shown are density plots of isolated platelets stained with FITC-CD41of the patient's mother (top panel) and the patient (bottom panel). Note the decreased mean forward scatter of the patient's platelets relative to those of her mother. (B) Western blots stained with WASP W-485 antibody. Shown are PBMCs (top panel) and platelets (bottom panel) of the (1) patient's mother, (2) the female patient, and (3) PBMCs of an unrelated typical WAS patient (exon 2; G252A).