Fig. 4.
Fig. 4. Peptide specificity of P1218-specific CTLs. / (A) Representative experiment showing cytotoxicity of P1218-specific CTL lines 1 to 3 against T2 cells alone, pulsed with P1218 or with a control HLA-A0201 binding peptide. Results are shown as the means ± SDs of triplicate wells. (B) Peptide titration of P1218-specific CTLs. Cytolytic activity of lines 1 to 3 against T2 cells pulsed with varying concentrations of P1218 is shown. Results are shown as the means ± SDs of triplicate wells. (C) Cold target inhibition of cytotoxic activity of P1218-specific CTLs. Cytotoxic activity of CTL line 2 against T2 cells pulsed with P1218 or control peptide in the presence or absence of a 50-fold excess of unlabeled K562 cells. Results are shown as the means ± SDs of triplicate wells.

Peptide specificity of P1218-specific CTLs.

(A) Representative experiment showing cytotoxicity of P1218-specific CTL lines 1 to 3 against T2 cells alone, pulsed with P1218 or with a control HLA-A0201 binding peptide. Results are shown as the means ± SDs of triplicate wells. (B) Peptide titration of P1218-specific CTLs. Cytolytic activity of lines 1 to 3 against T2 cells pulsed with varying concentrations of P1218 is shown. Results are shown as the means ± SDs of triplicate wells. (C) Cold target inhibition of cytotoxic activity of P1218-specific CTLs. Cytotoxic activity of CTL line 2 against T2 cells pulsed with P1218 or control peptide in the presence or absence of a 50-fold excess of unlabeled K562 cells. Results are shown as the means ± SDs of triplicate wells.

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