Fig. 6.
Fig. 6. Interaction of recombinant human protein S with Ca2+-dependent monoclonal antibodies. / Antibodies were coated into the wells of microtiter plates at 10 μg/mL and blocked with casein. Dialyzed recombinant protein S at a range of concentrations was incubated for 1 hour at 37°C in the presence of 3 mM CaCl2. Bound protein S was detected by means of a rabbit anti–human protein S peroxidase-conjugated antibody. (A) HPS21; (B) HPS54. In both panels, results are expressed as mean A492nm of duplicates as a proportion of the maximum (designated 100%). Each of the duplicates gave essentially identical results. ▪ indicates wild-type protein S; ●, protein S with Gly11Asp; ▴, protein S with Thr37Met.

Interaction of recombinant human protein S with Ca2+-dependent monoclonal antibodies.

Antibodies were coated into the wells of microtiter plates at 10 μg/mL and blocked with casein. Dialyzed recombinant protein S at a range of concentrations was incubated for 1 hour at 37°C in the presence of 3 mM CaCl2. Bound protein S was detected by means of a rabbit anti–human protein S peroxidase-conjugated antibody. (A) HPS21; (B) HPS54. In both panels, results are expressed as mean A492nm of duplicates as a proportion of the maximum (designated 100%). Each of the duplicates gave essentially identical results. ▪ indicates wild-type protein S; ●, protein S with Gly11Asp; ▴, protein S with Thr37Met.

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