Cytofluorometric analysis of Fas-receptor expression on spleen CD4 and CD8 lymphocytes from mice injected with CHO or hMOR/CHO cells.

Mice injected with recombinant hMOR/CHO cells in which splenocytes were not sensitized to Fas-mediated apoptosis were excluded from the analysis. Spleen cells from BALB/c mice injected with either untransfected CHO cells or recombinant hMOR/CHO cells were stained by using FITC-labeled anti-CD4 or anti-CD8 antibodies together with either PE-conjugated anti-CD95 or anti-KLH antibodies. Data were analyzed for Fas expression gated on either CD4 (A) or CD8 (B) T lymphocytes. The panel on the left shows a representative Fas staining on CD4 (top) and CD8 (bottom) T lymphocytes from CHO-injected mice (open histogram) and hMOR/CHO-injected mice (filled histogram). Histograms in the right panel show, for CHO-injected mice (n = 5; ■) and hMOR/CHO-injected mice (n = 3; ░), the corresponding means ± SD of the MFI.