Binding activity of anti-hMOR EL1 IgG and anti-hMOR EL3 IgG raised in mice.

The IgG binding activity toward the hMOR EL1 peptide (A) and the hMOR EL3 peptide (C) in pooled serum from mice immunized against the hMOR EL1 peptide (■) and pooled serum from mice immunized against the hMOR EL3 peptide (○) was estimated by enzyme-linked immunosorbent assay. A representative result from 3 experiments performed in triplicate is shown. The panels on the right show the inhibition of the binding activity of anti-hMOR EL1 IgG (B) and anti-hMOR EL3 IgG (D) by peptides corresponding to the human MOR EL1 sequence (NYLMGWPFGTILCK), to an analog of the murine MOR EL1 sequence (NYLMGWPFGAILCK), to the human MOR EL3 sequence (KALVTIPETTFQT), and to the murine MOR EL3 sequence (KALITIPETTFQT). Results are expressed as means ± SD percentage of inhibition of IgG binding in the presence of peptide at a concentration of 250 μg/mL. At least 3 independent experiments were performed in triplicate. O.D. indicates optical density.