Fig. 9.
Fig. 9. Splenocytes from mice that underwent passive transfer with both anti-hMOR EL1 IgG and anti-hMOR EL3 IgG are sensitized to CD95-mediated apoptosis. / Two hundred micrograms of either normal IgG (⋄), anti-hMOR EL1 IgG (○), anti-hMOR EL3 IgG (▵), or anti-hMOR EL1 IgG (100 μg) together with anti-hMOR EL3 IgG (100 μg) (■) were transferred by intravenous injection into naive BALB/c mice, which were killed 24 hours later. Shown is the percentage of subdiploid nuclei when splenocytes were incubated in the presence of anti-Fas IgG mAb Jo2 for 24 hours. Nuclei DNA peak was analyzed by cytofluorometry after incubation of the cells in hypotonic medium containing propidium iodide. Each experiment was performed in triplicate. Basal apoptosis of splenocytes in the absence or presence of hamster anti-KLH IgG mAb was 18% ± 3%. Statistical analysis was performed with the Mann-Whitney U test.

Splenocytes from mice that underwent passive transfer with both anti-hMOR EL1 IgG and anti-hMOR EL3 IgG are sensitized to CD95-mediated apoptosis.

Two hundred micrograms of either normal IgG (⋄), anti-hMOR EL1 IgG (○), anti-hMOR EL3 IgG (▵), or anti-hMOR EL1 IgG (100 μg) together with anti-hMOR EL3 IgG (100 μg) (■) were transferred by intravenous injection into naive BALB/c mice, which were killed 24 hours later. Shown is the percentage of subdiploid nuclei when splenocytes were incubated in the presence of anti-Fas IgG mAb Jo2 for 24 hours. Nuclei DNA peak was analyzed by cytofluorometry after incubation of the cells in hypotonic medium containing propidium iodide. Each experiment was performed in triplicate. Basal apoptosis of splenocytes in the absence or presence of hamster anti-KLH IgG mAb was 18% ± 3%. Statistical analysis was performed with the Mann-Whitney U test.

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