Fig. 2.
Syk is required for the integrin-dependent, but not the integrin-independent, component of the fMLP-induced respiratory burst in adhesion.
Wild-type or syk−/− neutrophils were plated on a fibrinogen-coated surface either in the presence or absence of Mg2+ salts and stimulated with 50 ng/mL TNF (A) or 3 μM fMLP (B) at zero time point. Background superoxide release in nonstimulated samples has been subtracted. Since Mg2+ ions are essential for the function of integrins, wild-type values in the absence of Mg2+ represent the integrin-independent component of the respiratory burst.
