Detection of the FLT3-LM in exon 20 in AML.

(A) Clinical data and alterations of other molecular markers in the 2 patients carrying the FLT3-840GS mutation. FLT3-LM indicates length mutations in the JM region of FLT3; FLT3D835, mutations in codons 835/836; KITD816, point mutations in codon 816; NRAS, activating point mutations in codons 12, 13, and 61; MLL-PTD, MLL-partial tandem duplication. (B) Detection of the LM in exon 20 after conventional agarose gel electrophoresis of PCR products from cDNA and gDNA. M indicates molecular weight standard; H₂O, water control; C, patient without exon 20 mutation; P1 and P2, patients 1 and 2, respectively, with the length mutation in exon 20. (C) Confirmation of the mutation by BamHI digestion after polyacrylamide gel electrophoresis. M indicates molecular weight standard; MT, patient with mutation; WT, 2 patients without mutation; H₂O, water control. (D) Schematic presentation of the FLT3-840GS mutation.