American Society of Hematology

Fig. 4.

$Fig. 4. Demonstration of p27Kip1/Skp2 complexes in nuclear and cytoplasmic fractions. / Immunoprecipitation studies demonstrate p27Kip1/Skp2 complexes within nuclear and cytoplasmic fractions. Subcellular fractions of Jurkat, CEM-6, Raji, and Nalm-6 cells were immunoprecipitated with polyclonal antibody to Skp2 followed by Western blot analysis with anti-p27Kip1. Subcellular fractions analyzed with antibody to Ki-67 show that expression of this protein was restricted to nuclear fractions. Western blot analysis for p27Kip1, however, demonstrates in vitro complexes of p27Kip1/Skp2 within both the cytoplasmic and the nuclear fractions.$

Demonstration of p27^Kip1/Skp2 complexes in nuclear and cytoplasmic fractions.

Immunoprecipitation studies demonstrate p27^Kip1/Skp2 complexes within nuclear and cytoplasmic fractions. Subcellular fractions of Jurkat, CEM-6, Raji, and Nalm-6 cells were immunoprecipitated with polyclonal antibody to Skp2 followed by Western blot analysis with anti-p27^Kip1. Subcellular fractions analyzed with antibody to Ki-67 show that expression of this protein was restricted to nuclear fractions. Western blot analysis for p27^Kip1, however, demonstrates in vitro complexes of p27^Kip1/Skp2 within both the cytoplasmic and the nuclear fractions.

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