Mice that received transplants of FasL+ allogeneic lin− BMs had enhanced engraftment early after BMT.
(A) B6.SJL (CD45.1+) lin− BMs were transduced with either control GFP or FasL(-GFP) vector. Cells were analyzed by FACS to determine the level of GFP fluorescence of the transduced cells prior to transplantation. (B) 105transduced lin− BM cells were transplanted intravenously into 400-cGy irradiated C3H.SW (CD45.2+) recipients. After mice were killed at 3 weeks after BMT, mouse organs were analyzed by FACS for correlated expression of CD45.1 and GFP. The graph shows the compilation of data for all mice shown in Table 1, with the average percentage of CD45.1+ cells in BM of each group. (C) The figure shows a representative plot for CD45.1 for a mouse that received a transplant of GFP-modified cells (top) and a mouse that received a transplant of FasL-modified cells (bottom). (D) These histograms show the GFP fluorescence of gated CD45.1+(donor) cells (from panel B) of representative mice that received transplants of lin− BMs transduced with either control GFP or FasL and analyzed at 3 weeks after BMT.
