Mice that received transplants of FasL⁺lin⁻ BMs did not have hepatocellular injury or enhanced hepatic inflammation and retained immune responsiveness to a third-party alloantigen.

(A,B) Livers from mice shown in Figure 3were fixed in formaldehyde, cut into paraffin blocks, then stained with hematoxylin and eosin for evaluation of inflammation. Panel A is a representative section from a control mouse that underwent GFP transplantation, and panel B is from the liver of a representative mouse that underwent FasL⁺ transplantation. (C) Splenocytes from mice that underwent transplantation were incubated as responders with irradiated (3000 cGy) allogeneic third-party stimulator (BALB/c) spleen cells. Four days after adding stimulators,³H-thymidine was added overnight. Cells were then harvested, and incorporation of ³H-thymidine was determined. This panel shows the proliferative responses (group averages ± SEM) from 8 mice that received transplants of FasL-transduced lin⁻ BMs, 7 mice that received transplants of control GFP-transduced lin⁻ BMs, 4 mice that received transplants of untransduced lin⁻ BMs, and 3 control C3H.SW mice that did not undergo transplantation, taken from 3 separate experiments.