Cell surface staining of CAG cells with OPG.

Alexa Fluor 488–conjugated human OPG (green) and Alexa Fluor 594–conjugated CTB (red) colocalize (yellow) on the uropod of polarized CAG cells. The cells were incubated with labeled OPG or CTB or both for 30 minutes on ice, washed, and thereafter examined by confocal microscopy. Original magnification × 630..