Fig. 4.
Fig. 4. The effect of the inflammatory milieu from the lungs of CF patients on baseline pHi and pHi in phagocytosing neutrophils. / Isolated human peripheral blood neutrophils were exposed to CF ELF (concentrated from BAL fluid from stable CF patients or healthy controls), and pHi was measured. (A) The acute alkalinizing effect on pHi of the addition of CF ELF (40 μL/mL) to neutrophils in culture. (B) The effect of preincubation with CF ELF (30 minutes, 30 μL/mL) on pHi after exposure to opsonized heat-killed E coli (EC; pathogen-to-phagocyte ratio 20:1) (*P < .02, CF versus normal ELF by 2-way ANOVA). All data mean ± SEM.

The effect of the inflammatory milieu from the lungs of CF patients on baseline pHi and pHi in phagocytosing neutrophils.

Isolated human peripheral blood neutrophils were exposed to CF ELF (concentrated from BAL fluid from stable CF patients or healthy controls), and pHi was measured. (A) The acute alkalinizing effect on pHi of the addition of CF ELF (40 μL/mL) to neutrophils in culture. (B) The effect of preincubation with CF ELF (30 minutes, 30 μL/mL) on pHi after exposure to opsonized heat-killed E coli (EC; pathogen-to-phagocyte ratio 20:1) (*P < .02, CF versus normal ELF by 2-way ANOVA). All data mean ± SEM.

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