mSIGNR1 binds both mouse ICAM-2 and HIV-1 gp120, similar to both hDC-SIGN and hL-SIGN.

(A) Polyclonal antibodies against mSIGNR1 stain 293T cells transfected with mSIGNR1 cDNA. 293T cells were transfected with different cDNAs and subsequently stained with antibodies against hDC-SIGN (AZN-D1; filled histogram) and mSIGNR1 (698; filled histogram). Isotype controls are shown as open histograms. One representative experiment of 3 is shown. (B) mSIGNR1 binds human ICAM-2, ICAM-3, and HIV-1 gp120 but not human ICAM-1, similar to hDC-SIGN and hL-SIGN. The adhesion of 293T cells transfected with mSIGNR1 to the ligands was determined using the fluorescent bead adhesion assay. Specificity was determined in the presence of blocking antibodies (AZN-D2, 20 μg/mL), mannan (5 μg/mL), or EGTA (5 mM). Cells were also preincubated with the polyclonal antibody against mSIGNR1 (1:10). One representative experiment of 3 is shown. (C) mSIGNR1 binds murine ICAM-2 with high affinity, similar to hDC-SIGN and hL-SIGN. The adhesion assay was performed as described in panel B. One representative experiment of 3 is shown.