mSIGNR1 mediates rapid internalization of captured dextran.

(A) Dextran is efficiently captured by mSIGNR1. K562 cells expressing mSIGNR1 and mock-transfected K562 cells were incubated with FITC-dextran (1 μg/mL) at 37°C and unbound dextran was washed away after 45 minutes. Specificity was determined in the presence of the antibody ER-TR9. Binding was analyzed by flow cytometry. SD was less than 5%. (B) Dextran is rapidly internalized by SIGNR1. The fate of bound FITC-dextran was followed by analyzing FITC-staining of K562 cells expressing mSIGNR1 after incubation with FITC-dextran using immunofluorescence microscopy. Original magnification × 200.