Allogeneic MLR induced by blood CD11c⁺ DCs, Mo-DCs and ActMo-DCs.

(A-I) Increasing numbers of blood CD11c⁺ DCs (▴), Mo-DCs (generated in RPMI/5% ABS or AS, GM-CSF 200 U/mL, IL-4 50 U/mL) (●), or ActMo-DCs (activated with LPS) (○) were prepared from the same donor and cultured with 1 × 10⁵ allogeneic CD3⁺ lymphocytes (DC/T ratio; 1:10-1000). (J) Increasing numbers of blood CD11c⁺ DCs (▴), Mo-DCs generated in RPMI/5% AS GM-CSF 200 U/mL, IL-4 50 U/mL (●), and ActMo-DCs activated with LPS (○) or with MCM (■) were prepared from the same donor and cultured with 1 × 10⁵ allogeneic CD3⁺ lymphocytes (DC/T ratio; 1:10-1000). (K) Increasing numbers of blood CD11c⁺ DCs (▴), Mo-DCs generated in RPMI/1% AS, GM-CSF 1000 U/mL, IL-4 1000 U/mL (●), and ActMo-DCs activated with LPS (○) or with MCM (■) were prepared from the same donor and cultured with 1 × 10⁵ allogeneic CD3⁺ lymphocytes (DC/T ratio; 1:10-1000). Results shown are presented as the mean ± SD cpm of triplicate wells.