TT-specific proliferative responses induced by blood CD11c⁺ DCs, Mo-DCs, and ActMo-DCs.

TT-specific T-lymphocyte responses induced by a graded number (2500/well-10 000/well) of blood CD11c⁺ DCs (▴), Mo-DCs (produced in RPMI/5% AS, GM-CSF 200 U/mL, and IL-4 50 U/mL) (●) or ActMo-DCs (produced in RPMI/5% AS, GM-CSF 200 U/mL, and IL-4 50 U/mL and activated with LPS) (○) loaded with 0 μg/mL to 10 μg/mL TT for 2 hours. CD3⁺ lymphocytes (1 × 10⁵/well) from TT known responders were used in A through D (DC/T ratio; 1:10-40), while TT-specific T-cell clones (5 × 10⁴/well) were used in E and F (DC/T ratio; 1:5-40). Graphs A and B related to case 2, C and D to case 5, and E and F to case 12. Results are presented as the mean ± SD cpm of triplicates.