IFN-γ production in T lymphocytes in the allogeneic MLR induced by blood CD11c⁺ DCs, Mo-DCs, and ActMo-DCs.

Allogeneic CD45RA⁺CD4⁺ T-lymphocyte proliferation and intracellular IFN-γ production induced by 1 × 10⁴ (A) blood CD11c⁺ DCs, (B) Mo-DCs and ActMo-DCs (produced in RPMI/5% AS, GM-CSF 200 U/mL, IL-4 50 U/mL and activated with LPS or MCM), (C) Mo-DCs and ActMo-DCs (produced in RPMI/1% AS, GM-CSF 1000 U/mL, IL-4 1000 U/mL and activated with LPS or MCM) (DC/T ratio; 1:10). The percentages of T lymphocytes producing IFN-γ are indicated on the dot plots. One representative experiment from the 3 experiments performed is shown.