Cytogenetic and molecular characterization of the SAM-1 and K-562 cell lines.

SKY analysis of SAM-1 (A) and K-562 (B) cells. Translocations are identified with numbers, and structural abnormalities (observed by G-banding) are indicated with arrows. See text for complete description. (C) Using SAM-1 cells, dual-color FISH probe forBCR, (green signal), was seen on 22q11 on 2 normal chromosomes 22, ABL, (red signal), was seen on the 9q34 band on the del(9)(p13) and on both ends of der(9)t(9;9)(p1?3;q22). Simple fusion signals were observed in Ph chromosome and in add(2). Multiple fusion signals were seen in the der(13) and in the der(22). (D) PCR-based analysis of 8 dinucleotide repeat loci in 6 myeloid leukemia cell lines. The D20S889 marker is on p-arm, whereas all others are on the q-arm of chromosome 20. SAM-1 and K562 cells exhibit an identical pattern of PCR amplification.