Gfi-1B does not affect erythroid differentiation.

CD34⁺ cells were transduced with empty vector (control),Gfi-1B or Bcl-xL, or cotransduced withGfi-1B and Bcl-xL. Cells were cultured in the presence of indicated cytokines. With exogenous Bcl-xL, a small but significant number of erythroblasts developed even in the absence of EPO (data not shown). On day 10 of culture, glycophorin A⁺ erythroblasts were purified by cell sorting, and differentiation was evaluated by benzidine staining.