Fig. 4. Methylation changes of the 5′... | American Society of Hematology

Fig. 4.

$Fig. 4. Methylation changes of the 5′ p15 gene during decitabine treatment visualized by DGGE. / (A) Bone marrow cells of patient 019 before and after courses 1, 2, and 6 of DAC treatment were analyzed by DGGE as described in “Patients, materials, and methods.” The PCR reaction was initiated by “hot start,” followed by 39 cycles at 94°C for 20 seconds, 55°C for 20 seconds, and 72°C for 30 seconds, and a final extension at 72°C for 5 minutes. Normal PBCs and in vitro methylated DNA served as negative and positive controls, respectively. Methylated alleles migrate farther than unmethylated alleles. The percentage of bone marrow blasts (relative to the nonerythroid cell fraction) is given below each lane. (B) Bone marrow cells of patient 013 before treatment and after courses 1 and 2 were analyzed by DGGE as described above.$

Methylation changes of the 5′ p15 gene during decitabine treatment visualized by DGGE.

(A) Bone marrow cells of patient 019 before and after courses 1, 2, and 6 of DAC treatment were analyzed by DGGE as described in “Patients, materials, and methods.” The PCR reaction was initiated by “hot start,” followed by 39 cycles at 94°C for 20 seconds, 55°C for 20 seconds, and 72°C for 30 seconds, and a final extension at 72°C for 5 minutes. Normal PBCs and in vitro methylated DNA served as negative and positive controls, respectively. Methylated alleles migrate farther than unmethylated alleles. The percentage of bone marrow blasts (relative to the nonerythroid cell fraction) is given below each lane. (B) Bone marrow cells of patient 013 before treatment and after courses 1 and 2 were analyzed by DGGE as described above.

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