Flow cytometric analysis of Crry expression.

Stably transfected populations of human tumor cell lines expressing rat Crry were isolated by several rounds of cell sorting. Figure shows analysis of sorted populations used in experiments (A-C). Endogenous expression of Crry on 13762 rat mammary tumor cells is also shown (D). Cells were stained by immunofluorescence using anti–rat Crry monoclonal antibody 5I2. Untransfected MCF7 cells are shown as control, but the same levels of fluorescence were seen with other untransfected cell lines.