Expression of rat Crry on MCF7 cells inhibits rat cell–mediated cytoxicity.
MCF7 cells and Crry transfected MCF7 cells were exposed to either a B-cell–depleted rat splenocyte preparation at an E/T ratio of 100:1 (panel A) or to purified rat NK cells at various E/T ratios (panel B). In the experiment shown in panel A, target cells were preincubated in the presence of either anti–MCF7 antibody, C6-deficient rat serum complement source, both antibody and complement, or PBS. Panel A also shows the effect of NK cell depletion on cytolytic activity of the splenocyte preparation. In the experiment shown in panel B, target cells were preincubated with either anti–MCF7 antibody or PBS as indicated. Lysis was determined by 51Cr release assay after a 4-hour incubation. Shown are the experimental means ± SD (n = 3); data are representative of 3 (panel A) or 2 (panel B) separate experiments.
