Fig. 3.
Expression of rat Crry on SKBR3 and LAN1 tumor cells inhibits NK-mediated antibody-dependent cell cytotoxicity.
Control transfected or Crry-transfected SKBR3 cells (A) or LAN1 cells (B) were preincubated either in the presence or absence of target cell–specific antibody as indicated (either anti–SKBR3 membrane polyclonal antibody or anti–GD2 mAb for LAN-1 cells). Cells were then washed and exposed to purified rat NK cells. Lysis was determined by51Cr release assay after a 4-hour incubation. Figures show means ± SD (n = 3) and are representative of 2 (panel A) or 4 (panel B) experiments.
