Fig. 4.
Anti–Crry F(ab)2 fragments reverse the inhibitory effect of Crry expressed on MCF7 cells.
Parental MCF7 or Crry-transfected MCF7 cells were preincubated with PBS or the indicated concentration of anti–Crry F(ab)2. Cells were then incubated either in the absence or presence of anti–MCF7 polyclonal antibody, washed and used as target cells in cytolysis assays with rat splenocytes enriched for NK cells as effector cells (E/T ratio of 100:1). Lysis was determined by 51Cr release assay after a 4-hour incubation. Data shown are representative of 6 separate experiments.
