Fig. 5.
Anti–Crry F(ab)2 fragments reverse the inhibitory effect of Crry expressed on LAN1 cells.
Target LAN1 or Crry-transfected LAN1 cells were preincubated with PBS or 10 μg/mL anti–Crry F(ab)2. Cells were then incubated either in the absence or presence of anti–GD2 monoclonal antibody as indicated, washed and tested for susceptibility to lysis by enriched NK cells (E/T ratio of 100:1). Lysis was determined by 51Cr release assay after a 4-hour incubation. Figure shows means ± SD (n = 3) and is representative of 5 separate experiments.
