Fig. 5.
Effect of cycloheximide on hypoxia-mediated inhibition of neutrophil apoptosis.
Isolated neutrophils were cultured at 5 × 106/mL in the presence or absence of 50 μg/mL (A) or 0.1 to 10 μg/mL (B) of CHX in atmospheres containing 21% or 3% oxygen. Cells were recovered at the time points indicated, and apoptosis was assessed morphologically. (A) Time-course data (CHX 50 μg/mL) showing mean ± SEM of triplicate incubations from a single experiment representative of 2. (B) Concentration-response data at 20 hours (open bars, cells incubated in 21% oxygen; gray bars, cells incubated at 3% oxygen). Data represent mean ± SEM of 6 separate experiments.
