Fig. 7.
Effect of the iron chelators DFO and CP-94 on neutrophil apoptosis.
(A) Neutrophils were cultured at 5 × 106 cells/mL under normoxic (21% oxygen) conditions for 20 hours in the presence or absence of increasing concentrations of DFO or CP-94. (B) Cells were incubated again under normoxic conditions for 20 hours in the absence or presence of CP-94 (300 μM), DFO (300 μM), and FeCl2(3 mM) as indicated. Apoptosis was assessed morphologically. In panels A and B, data represent the mean ± SEM of 3 separate experiments, each performed in triplicate, with values in panel A expressed as a percentage of the matched-vehicle control values.
