Fig. 10.
Effect of caspase inhibition on neutrophil apoptosis.
(A) Neutrophils (5 × 106/mL) were cultured for 2 hours in the presence or absence of gliotoxin and TNF-α with or without 100 μM ZVAD-fmk. Data represent 6 replicates from one experiment. (B,C) Neutrophils (5 × 106/mL) were cultured under normoxic (21%), hypoxic (3%), or anoxic (0%) conditions for 6 hours (B) or 20 hours (C) in DMEM containing 10% autologous serum (Monofeed) in the presence or absence of 100 μM ZVAD-fmk or 25 μM Boc-D-fmk. Control, untreated, and 1 μL/mL dimethyl sulfoxide (vehicle)–treated neutrophils were also analyzed. Data represent the mean ± SEM of 3 experiments, each performed in triplicate.
