Fig. 5.
![Fig. 5. Effect of hyperhomocysteinemia on LDL uptake. / Mouse peritoneal macrophages were incubated with 20 μg/mL [125I]Ac-LDL or [125I]native-LDL for 2 hours. Protein-associated [125I]radioactivity was measured and normalized as [125I] counts per milligram of protein. The uptake activity of [125I]Ac-LDL in macrophages from CBS−/+/ApoE−/− mice under HF diet was set as the control. LDL uptake activity is expressed as relative cellular [125I]protein (% of control mean values). (Inset) The image of LDLs on 0.75% agarose gel shows the electrophoretic mobility change by acetylation. Values represent mean ± SD from 3 independent assays (n = 9). *P < .01 versus CBS+/+/ApoE−/−; †P < .01 versus CBS−/+/ApoE−/− in the same group.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/10/10.1182_blood-2002-08-2606/4/h81034332005.jpeg?Expires=1735839298&Signature=24M8-RrPr7CGpjUhtsVsueThR478tIbooYNPwj~J0z6SReJ7zeRbWeIK-NOQ7062OaOxHlb8~Xi2nEcdusFo2P3OLNG5Cv~zLhyHIsXeNsdICo2qzdtfcypQeQIKG4XHLqQLilO0tcPUKvq8IyCPf1ecabHQta0Kd4eCfJOVgomYDCJ45VVWwjlxxu~ZL9b-JYxYRqbBECQXGKE7WQaO3wv7o8cnaRC5JRUltAYnMOd9Y6h4q-dAGftEqg6z8ixGysdKSZkPOEb466zBmmPHoxCxXIrrouBpKC50EJtWMH-BiHB1FmRXLpa0lkHyqMJgRNaGvMxj9ustKjP6MBY0JA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of hyperhomocysteinemia on LDL uptake.
Mouse peritoneal macrophages were incubated with 20 μg/mL [125I]Ac-LDL or [125I]native-LDL for 2 hours. Protein-associated [125I]radioactivity was measured and normalized as [125I] counts per milligram of protein. The uptake activity of [125I]Ac-LDL in macrophages from CBS−/+/ApoE−/− mice under HF diet was set as the control. LDL uptake activity is expressed as relative cellular [125I]protein (% of control mean values). (Inset) The image of LDLs on 0.75% agarose gel shows the electrophoretic mobility change by acetylation. Values represent mean ± SD from 3 independent assays (n = 9). *P < .01 versus CBS+/+/ApoE−/−; †P < .01 versus CBS−/+/ApoE−/− in the same group.
