Fig. 1.
Fig. 1. Thymic expression of FGFR2IIIb. / (A) RT-PCR analysis of cDNA prepared from whole thymi of indicated ages. Samples were normalized for HPRT expression using a constant amount of HPRT competitor cDNA (lower panel). Normalized samples were then analyzed with FGFR2IIIb primers (upper panel). Thymocyte sample was prepared from a pool of day-16 fetal thymi. Representative of 4 independent experiments. (B) Detection of FGFR2IIIb in RT-PCR analysis of the TE-71 thymic epithelial cell line. Representative of 2 independent experiments. (C) In situ hybridization localization of FGFR2IIIb message in young adult thymus. Arrow denotes rim of labeling associated with the subcapsular epithelium. C indicates cortex; M, medulla. Magnification × 40. Representative of 3 independent experiments. (D) Immunohistochemical localization of FGFR2 protein in young adult thymus. C indicates cortex; M, medulla. Magnification × 40. Representative of 2 independent experiments.

Thymic expression of FGFR2IIIb.

(A) RT-PCR analysis of cDNA prepared from whole thymi of indicated ages. Samples were normalized for HPRT expression using a constant amount of HPRT competitor cDNA (lower panel). Normalized samples were then analyzed with FGFR2IIIb primers (upper panel). Thymocyte sample was prepared from a pool of day-16 fetal thymi. Representative of 4 independent experiments. (B) Detection of FGFR2IIIb in RT-PCR analysis of the TE-71 thymic epithelial cell line. Representative of 2 independent experiments. (C) In situ hybridization localization of FGFR2IIIb message in young adult thymus. Arrow denotes rim of labeling associated with the subcapsular epithelium. C indicates cortex; M, medulla. Magnification × 40. Representative of 3 independent experiments. (D) Immunohistochemical localization of FGFR2 protein in young adult thymus. C indicates cortex; M, medulla. Magnification × 40. Representative of 2 independent experiments.

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