KGF effects on thymic lobes depleted of hematogenous elements.

(A) DOG-treated thymic lobes were cultured in medium (i, iii, v, vii) or KGF (ii, iv, vi, viii) and then processed to demonstrate expression of E-cadherin (i, ii), cortical epithelium (iii, iv), medullary epithelium (v, vi), or MHC class II antigens (vii, viii). Cystic structures are indicated by asterisks, and the medullary TE foci in the medium-treated FTOC are indicated by arrowheads. Representative of 2 separate experiments, each with 5-7 lobes per condition. Panels i-viii, magnification × 45. (B) RT-PCR analysis of cytokine expression in medium and KGF-treated DOG lobes. Analysis of serial 3-fold dilutions of cDNA samples prepared from medium or KGF-treated DOG lobes (previously normalized for HPRT expression).