Western blot analysis of YY1 expression in 32D-WT1 cells.

G-CSF responsive 32D-WT1 cells were cultured in IL-3–containing medium and then switched to G-CSF. Samples were taken daily and processed as described in “Materials and methods.” The blot was hybridized with anti-YY1 or anti-Sp1, stripped, and rehybridized with anti-Actin to check for equal loading of cell lysates.